5-(Ethylthio)-1H-tetrazole (ETT)

ETT acts as a high-efficiency coupling activator in oligonucleotide synthesis, ensuring rapid and complete bond formation between nucleoside units. This improves yield and purity in the phosphonamidite method.

In RNA and ribozyme synthesis, ETT serves as an effective phosphonamidite activator, improving coupling efficiency during solid-phase synthesis. The resulting high-quality RNA strands are vital for mRNA vaccine production, enabling rapid response to infectious diseases, and for CRISPR tools, providing precise RNA.

ETT functions as a solid-phase coupling agent for producing symmetrical dinucleotides with high purity and accuracy. These are widely used in molecular biology to study nucleic acid structures, enzyme interactions, and sequence-specific binding.

ETT is applied in specialized click chemistry workflows, particularly in high-value bioconjugation and solid-phase synthesis, where it boosts reaction speed and yield.